科研之友
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摘要
Outbreaks of infectious diseases among freshwater fish damage the aquaculture industry. A technique for detecting pathogens in environmental water samples has recently been developed. In the present work, the monitoring of pathogenic viruses in Lake Erhai and Lake Dianchi (Yunnan, China) was performed. Yunnan possesses a rich diversity of cyprinid fish, and the aquaculture of freshwater fish is a highly prosperous industry in this region. Thus, Cyprinid herpesvirus 1, 2, and 3 as well as three rhabdoviruses, Infectious hematopoietic necrosis virus, Viral hemorrhagic septicemia virus, and the Spring viremia of carp virus, all of which have severe impacts on aquaculture, were targeted. Viruses in lake surface water were concentrated and then quantified/detected by real-time PCR/real-time reverse transcription PCR. Five of the six tested viruses were detected in lake water. Although there has been no reported viral outbreak in the survey lakes, our results indicate a potential risk for these viral diseases.
