Neuroprotective effects of LBP on brain ischemic reperfusion neurodegeneration

作者：Wang, H. -B; Li, Y. -X.; Hao, Y. -J.; Wang, T. -F.; Lei, Z.; Wu, Y.; Zhao, Q. -P.; Wang, H.; Ma, L.; Liu, J.; Zhao, C. -J.; Jiang, Y. -X.; Wang, Y. -R.; Dai, X. -Y.; Zhang, W. -N.; Sun, T.; Yu, J. -Q.^*

来源：European Review for Medical and Pharmacological Sciences, 2013, 17(20): 2760-2765.

• LBP
• Focal cerebral ischemia/reperfusion
• Neuroprotection
• ROS

AIM: The present study was conducted to investigate whether LBP had a protective effect on cerebral ischemic reperfusion injury and to determine the possible mechanisms.
MATERIALS AND METHODS: Male Kunming (KM) mice were used to make the model cerebral artery occlusion/reperfusion (MCAO/R). The behavioral test was used to measure neurological deficit scores for evaluation of ischemic reperfusion damage of brain. The change of electroencephalograph (EEG) was monitored by Model SMUP-E Bio-electric Signals Processing System. The infarction area of brain was assessed in brain slices with 2% solution of 2,3,5-triphenyl tetrazolium chloride (TTC). Spectrophotometric assay was used to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and lactate dehydrogenase (LDH), contents of malondialdehyde (MDA) and adenosine triphosphate (ATP) of the brain.
RESULTS: The results showed that LBP at doses of 20 and 40 mg/kg markedly decreased the neurological deficit scores and the infarction area in MCAO/R mice. At the same time, LBP significantly decreased MDA content, and increased SOD, GSH-Px, CAT, LDH activities in ischemic reperfusion brain.
CONCLUSIONS: These suggest that LBP might act as a potential neuroprotective agent against the cerebral reperfusion-induced injury in the brain through reducing lipid peroxides, scavenging free radicals, and improving the energy metabolism.

• 出版日期2013-10
• 单位宁夏医科大学