Differential interference contrast microscopy for cells using hard x-ray holography

作者：Matsuda K; Lopez J C A; Rehman S; Misawa M^*; Suzuki Y; Takeuchi A; Yasumoto M; Hibino K; Roy M; Hanayama R; Ishii K

来源：Applied Optics, 2018, 57(17): 4795-4801.

DOI：10.1364/AO.57.004795

We propose a differential interference contrast method for cells using hard x-ray Gabor holography and knife-edge filtering in the spatial frequency domain, without relying on beam shearing. A phase object is holographically recorded and reconstructed by computer. Interference between the wavefronts of zeroth order weighted by e(j pi/2) in the positive frequency region produces a dark image. Similarly, interference between the wavefronts of the zeroth order weighted by e(j3 pi/2) in the negative frequency region produces a bright image. By adding these two intensity distributions, good quality phase-contrast images of 8-mu m-diameter polystyrene beads and human HeLa cells were obtained.

• 出版日期2018-6-10