hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis for species identification and differentiation of Mycobacterium kansasii and Mycobacterium chelonae-Mycobacterium abscessus group

作者：Sajduda Anna^*; Martin Anandi; Portaels Francoise; Palomino Juan Carlos

来源：International Journal of Infectious Diseases, 2012, 16(3): E193-E197.

DOI：10.1016/j.ijid.2011.11.011

• Mycobacterium kansasii
• Mycobacterium chelonae-Mycobacterium
• abscessus group
• hsp65
• PRA
• Identification
• Differentiation

Objectives: The aim of the present study was to identify and differentiate Mycobacterium kansasii and Mycobacterium chelonae-Mycobacterium abscessus group strains isolated from clinical and environmental sources in different countries.
Methods: PCR-restriction analysis of the hsp65 gene (PRA) with automated capillary electrophoresis was applied to the isolates previously identified by conventional biochemical testing and the molecular INNO-LiPA MYCOBACTERIA assay.
Results: PRA performed very well in comparison with the two other methods (96.4% concordance). Among 27 M. kansasii isolates, this method detected five genetic types, of which type 1 represented the most common clinical isolate, as it is worldwide. PRA differentiated 29 M. chelonae-M. abscessus group isolates into Mycobacterium immunogenum type 2 (n = 13), M. chelonae (n = 12), and M. abscessus types 1 (n = 1) and 2 (n = 1). M. immunogenum was the most frequent (69%) isolate from humans, but only one of 11 cases was clinically significant. M. chelonae was the most commonly (83%) recovered from water. PRA also identified two isolates with hsp65 alleles representing previously unreported patterns.
Conclusions: PRA based on automated capillary electrophoresis is a rapid, simple, and reliable method for the identification and differentiation of both clinically relevant and environmental isolates of M. kansasii and M. chelonae-M. abscessus group.

• 出版日期2012-3