Pyrrolysine Analogs as Substrates for Bacterial Pyrrolysyl-tRNA Synthetase in Vitro and in Vivo

作者：Katayama Hidekazu^*; Nozawa Kayo; Nureki Osamu; Nakahara Yoshiaki; Hojo Hironobu

来源：Bioscience Biotechnology and Biochemistry, 2012, 76(1): 205-208.

DOI：10.1271/bbb.110653

• Desulfitobacterium hafnience
• pyrrolysine
• pyrrolysyl-tRNA synthetase

Pyrrolysine-tRNA(Pyl) complex is produced by pyrrolysyl-tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA(Pyl) in vitro. In addition, the PylRS/tRNA(Pyl) pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA(Pyl) pair can be used in protein engineering technology.

• 出版日期2012-1