Mutual modulation between norepinephrine and nitric oxide in haemocytes during the mollusc immune response

作者：Jiang Qiufen; Zhou Zhi; Wang Lingling^*; Yang Chuanyan; Wang Jingjing; Wu Tiantian; Song Linsheng

来源：Scientific Reports, 2014, 4(1): 6963.

DOI：10.1038/srep06963

Nitric oxide (NO) is one of the most important immune molecules in innate immunity of invertebrates, and it can be regulated by norepinephrine in ascidian haemocytes. In the present study, the mutual modulation and underlying mechanism between norepinephrine and NO were explored in haemocytes of the scallop Chlamys farreri. After lipopolysaccharide stimulation, NO production increased to a significant level at 24 h, and norepinephrine concentration rose to remarkable levels at 3 h and 12 similar to 48 h. A significant decrease of NO production was observed in the haemocytes concomitantly stimulated with lipopolysaccharide and alpha-adrenoceptor agonist, while a dramatic increase of NO production was observed in the haemocytes incubated with lipopolysaccharide and beta-adrenoceptor agonist. Meanwhile, the concentration of cyclic adenosine monophosphate (cAMP) decreased significantly in the haemocytes treated by lipopolysaccharide and alpha/beta-adrenoceptor agonist, while the content of Ca2+ was elevated in those triggered by lipopolysaccharide and beta-adrenoceptor agonist. When the haemocytes was incubated with NO donor, norepinephrine concentration was significantly enhanced during 1 similar to 24 h. Collectively, these results suggested that norepinephrine exerted varied effects on NO production at different immune stages via a novel alpha/beta-adrenoceptor-cAMP/Ca2+ regulatory pattern, and NO might have a feedback effect on the synthesis of norepinephrine in the scallop haemocytes.

• 出版日期2014-11-7
• 单位中国科学院大学; 中国科学院海洋研究所