An automated purification method for archaeal and bacterial tetraethers in soils and sediments

作者：Sanchi Lise^*; Menot Guillemette; Bard Edouard

来源：Organic Geochemistry, 2013, 54: 83-90.

DOI：10.1016/j.orggeochem.2012.10.005

Isoprenoid and branched glycerol dialkyl glycerol tetraethers (GDGTs) are archaeal and bacterial polar lipids increasingly used as environmental biomarkers, and are studied in a wide range of settings: lacustrine and oceanic sediments, water column particulate organic matter, soils, peats, sedimentary rocks and extracts from archaeal and bacterial cultures. In paleoclimatology, for example, typical work on a sediment core of several tens of m consists of several hundreds to more than a thousand HPLC-MS (high performance liquid chromatography-mass spectrometry) analyses. The measurements therefore require purification steps from total lipid extracts. We propose an automated procedure for obtaining the GDGT core lipid fraction. We first evaluate both the yield and efficiency of the separation using different cartridges. We then compare the results from automated and %26quot;classical manual%26quot; procedures for a soil and a marine sediment, as well as for a sedimentary paleosequence.

• 出版日期2013-1