AMP-activated protein kinase-mediated expression of heat shock protein beta 1 enhanced insulin sensitivity in the skeletal muscle

作者：Yuan, Hairui; Wang, Tianyi; Niu, Yanmei; Liu, Xiaolei; Fu, Li^*

来源：FEBS LETTERS, 2017, 591(1): 97-108.

DOI：10.1002/1873-3468.12516

• AMP-activated protein kinase
• heat shock protein beta-1
• insulin resistance
• skeletal muscle

Activation of AMP-activated protein kinase (AMPK) has been viewed as an important target for the treatment of insulin resistance. Here, by proteomic analysis, we found that expression of heat shock protein beta-1 (HSPB1) was induced by the AMP analog 5-aminoimidazole-4-carboxamide 1-beta-D-ribofur-anoside in palmitate-induced insulin-resistant cells. Overexpression of AMPK alpha 2, or activation of AMPK alpha via acute/chronic exercise training, increased HSPB1 expression in the skeletal muscle. In AMPK alpha 2(-/-) mice, HSPB1 expression was downregulated in the quadriceps muscles. Exercise did not increase HSPB1 expression in AMPK alpha 2(-/-) mice. Moreover, overexpression of HSPB1 enhanced insulin sensitivity in palmitate-induced insulin-resistant cells and restored metabolic phenotypes associated with defective AMPK. Finally, HSPB1 was required for AMPK-mediated activation of the class IIa histone deacetylases and glucose uptake in the skeletal muscle. Our results demonstrate that AMPK-mediated HSPB1 expression enhanced insulin sensitivity in the skeletal muscle.

• 出版日期2017-1
• 单位天津医科大学