Arginine kinase of Litopenaeus vannamei involved in white spot syndrome virus infection

作者：Ma, Fang-fang; Liu, Qing-hui^*; Guan, Guang-kuo; Li, Chen; Huang, Jie

来源：Gene, 2014, 539(1): 99-106.

DOI：10.1016/j.gene.2014.01.047

• White spot syndrome virus
• VP14
• Arginine kinase
• Litopenaeus vannamei

Virus-host interaction is important for virus infection. White spot syndrome virus VP14 contains transmembrane and signal peptides domain, which is considered to be important for virus infection. Until now, the function of this protein remains undefined. In this study, we explored the interaction of VP14 with host cell.A new shrimp protein (arginine kinase of Litopenaeus vannamei, LvAK) is selected and its localization in shrimp cells is also confirmed. Cellular localization of LvAK protein in shrimp hemocytes showed that LvAK was primarily located at the periphery of hemocytes and was scarcely detectable in the nucleus. Tissue distribution indicated that arginine kinase gene was spread commonly in the tissues and was highly present in shrimp muscle tissue. The expression of LvAK mRNA in muscle was significantly up-regulated after WSSV stimulation. Indirect immunofluorescence assay showed that LvAK interacted with VP14 in WSSV-infected shrimp. Injection of LvAK protein enhanced the mortality of shrimp infected with white spot syndrome virus (WSSV). These results showed that LvAK is involved in WSSV infection. Future research on this topic will help to reveal the molecular mechanism of WSSV infection.

• 出版日期2014-4-10
• 单位中国水产科学研究院; 上海海洋大学