Nesterenkonia suensis sp nov., a haloalkaliphilic actinobacterium isolated from a salt pan

作者：Govender Lucretia; Naidoo Lureshini; Setati Mathabatha Evodia^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2013, 63: 41-46.

DOI：10.1099/ijs.0.035006-0

A Gram-positive, non-motile, non-spore-forming actinobacterium designated strain Sua-BAC020(T) was isolated from brine from Sua salt pan in Botswana. The strain was alkaliphilic and moderately halophilic, displaying optimal growth at 35-37 degrees C, pH 9 and 2.5% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain Sua-BAC020(T) belonged to the genus Nesterenkonia, sharing 96.2-99.0% sequence similarity with the type strains of recognized species within this genus. DNA-DNA hybridization with the type strains of species that showed the closest phylogenetic affiliation, Nesterenkonia xinjiangensis (16S rRNA gene sequence similarity, 98.9%), Nesterenkonia aethiopica (99.0%), Nesterenkonia halophila (97.5%), Nesterenkonia flava (97.4%) and Nesterenkonia halobia (97.2%), gave relatedness values of 10-45%. The peptidoglycan type of strain Sua-BAC020(T) was A4 alpha, L-Lys-Gly-D-Asp. Cells of the isolate contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and unidentified glycolipids as major polar lipids, MK-8, MK-9 and MK-7 were the predominant menaquinones, and the major fatty acids (%26gt;10%) were anteiso-C-15:0 and anteiso-C-17:0. The DNA G+C content of strain Sua-BAC020(T) was 64.8 mol%. Based on DNA-DNA hybridization, and physiological and biochemical tests, strain Sua-BAC020(T) is distinct from all recognized Nesterenkonia species, suggesting that this strain represents a novel species, for which the name Nesterenkonia suensis sp. nov. is proposed. The type strain is Sua-BAC020(T) (=DSM 22748(T)=NCCB 100309(T)).

• 出版日期2013-1