A low-cost integrated biosensing platform based on SiN nanophotonics for biomarker detection in urine

作者：Martens D; Ramirez Priego P; Murib M S^*; Elamin A A; Gonzalez Guerrero A B; Stehr M; Jonas F; Anton B^*; Hlawatsch N; Soetaert P; Vos R; Stassen A; Severi S; Van Roy W; Bockstaele R; Becker H; Singh M; Lechuga L M; Bienstman P^*

来源：Analytical Methods, 2018, 10(25): 3066-3073.

DOI：10.1039/c8ay00666k

We present a low-cost integrated nanophotonic lab-on-a-chip platform suitable for point-of-care (POC) biomarker analysis. The sensor chip included in the platform contains multiplexed Mach-Zehnder interferometers with an on-chip optical spectral analyser consisting of an arrayed-waveguide grating. The sensor chip is fabricated in silicon nitride material, which makes it compatible with consumer-electronics-grade sources and detectors, leading to the possibility of low-cost instrumentation. The nanophotonic sensor chip exhibits a detection limit of 6 x 10(-6) RIU (Refractive Index Units), which is in the same order of magnitude as the reported values for state-of-the-art evanescent wave sensors. The sensor chip is biofunctionalised with specific bioreceptors and integrated into a polymer microfluidic cartridge. The POC instrumentation platform contains optical excitation and read-out sub-systems and dedicated on-board software for real-time analysis of patient samples. To demonstrate the versatility of the platform, we present results both on the detection of an antigen related to tuberculosis directly in urine samples using a laboratory prototype and on the detection of a protein biomarker (CRP) related to inflammation using the integrated instrument.

• 出版日期2018-7-7