A short GC-rich sequence involved in deletion formation of cloned DNA in E-coli

作者：Sumegi A; Birko Z; Szeszak F; Vitalis S; Biro S^*

来源：Acta Biologica Hungarica, 1997, 48(3): 275-279.

• spontaneous deletion
• recombination
• direct repeat
• Gd-rich DNA
• recA independent

A spontaneous deletion probably caused by rec A independent recombination between short-direct repeat sequences was observed in pUC19 plasmid carrying a piece of Streptomyces genomic DNA after culturing in liquid medium. The deletion removed an unknown portion of the cloned DNA and the BamHI-EcoRI part of the multiple cloning site with an additional flanking 111 bp from the vector. At the junction a 13 bp GC-rich DNA sequence highly homologous to a known spontaneous deletion hotspot was found.

• 出版日期1997