A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation.

作者：Li Meng Amy^*; Amaral Paulo P; Cheung Priscilla; Bergmann Jan H; Kinoshita Masaki; Kalkan Tuzer; Ralser Meryem; Robson Sam; von Meyenn Ferdinand; Paramor Maike; Yang Fengtang; Chen Caifu; Nichols Jennifer; Spector David L; Kouxarides Tony; He Lin^*; Smith Austin^*

来源：eLife, 2017, 6: e23468.

DOI：10.7554/eLife.23468

Execution of pluripotency requires progression from the naive status represented by mouse embryonic stem cells (ESCs) to a state capacitated for lineage specification. This transition is coordinated at multiple levels. Non-coding RNAs may contribute to this regulatory orchestra. We identified a rodent-specific long non-coding RNA (lncRNA) linc1281, hereafter Ephemeron (Eprn), that modulates the dynamics of exit from naive pluripotency. Eprn deletion delays the extinction of ESC identity, an effect associated with perduring Nanog expression. In the absence of Eprn, Lin28a expression is reduced which results in persistence of let-7 microRNAs, and the up-regulation of de novo methyltransferases Dnmt3a/b is delayed. Dnmt3a/b deletion retards ES cell transition, correlating with delayed Nanog promoter methylation and phenocopying loss of Eprn or Lin28a. The connection from lncRNA to miRNA and DNA methylation facilitates the acute extinction of naive pluripotency, a pre-requisite for rapid progression from preimplantation epiblast to gastrulation in rodents. Eprn illustrates how lncRNAs may introduce species-specific network modulations.

• 出版日期2017-8-18