Alkaline protease from Thermoactinomyces sp RS1 mitigates industrial pollution

作者：Verma Amit; Ansari Mohammad W^*; Anwar Mohmmad S; Agrawal Ruchi; Agrawal Sanjeev

来源：Protoplasma, 2014, 251(3): 711-718.

DOI：10.1007/s00709-013-0559-y

• Alkaline protease
• Potato peels
• Solid-state fermentation
• Detergent additive
• Extractants
• Silver recovery
• External factors
• Enzyme activity

Proteases have found a wide application in the several industrial processes, such as laundry detergents, protein recovery or solubilization, prion degradation, meat tenderizations, and in bating of hides and skins in leather industries. But the main hurdle in industrial application of proteases is their economical production on a large scale. The present investigation aimed to exploit the locally available inexpensive agricultural and household wastes for alkaline protease production using Thermoactinomyces sp. RS1 via solid-state fermentation (SSF) technique. The alkaline enzyme is potentially useful as an additive in commercial detergents to mitigate pollution load due to extensive use of caustic soda-based detergents. Thermoactinomyces sp. RS1 showed good protease production under SSF conditions of 55 A degrees C, pH 9, and 50 % moisture content with potato peels as solid substrate. The presented findings revealed that crude alkaline protease produced by Thermoactinomyces sp. RS1 via SSF is of potential application in silver recovery from used X-ray films.

• 出版日期2014-5