A simplified erythromycin resistance cassette for Treponema denticola mutagenesis

作者:Goetting Minesky M Paula; Fenno J Christopher*
来源:Journal of Microbiological Methods, 2010, 83(1): 66-68.
DOI:10.1016/j.mimet.2010.07.020

摘要

The primary selectable marker for the genetic studies of Treponema denticola is a hybrid gene cassette containing both ermF and ermAM (ermB) genes. ErmB functions in Escherichia coli, while ErmF has been assumed to confer resistance in T. denticola. We demonstrate here that ErmB is sufficient for erythromycin selection in T. denticola and that the native ermB promoter drives ErmB expression.

  • 出版日期2010-10