A new actinomycete strain SK4-6 producing secondary metabolite effective against methicillin-resistant Staphylococcus aureus

作者：Laidi Rabah Forar^*; Sifour Mohammed; Sakr Mahmoud; Hacene Hocine

来源：World Journal of Microbiology and Biotechnology, 2008, 24(10): 2235-2241.

DOI：10.1007/s11274-008-9735-1

• actinomycete
• medium optimization
• Plackett-Burman design
• strain SK4-6
• Streptomyces

A new actinomycete strain designated SK4-6, was isolated. This organism exhibited strong activity against bacteria including methicillin-resistant Staphylococcus aureus and Micrococcus luteus, in addition to the causative agents of Candidiasis and Aspergillosis diseases, Candida albicans and Aspergillus species respectively. Morphological and chemical studies indicated that this organism belongs to the genus Streptomyces. Analysis of the 16S rRNA sequence of strain SK4-6 showed a high similarity, 99%, with S. qinlingensis. Optimization of cultural conditions was carried out using Plackett-Burman statistical design where seven variables were examined. Starch, pH and inoculum size showed a positive effect on the production of the active substances, however, sucrose, (NH(4))(2)SO(4) and yeast extract repress the production.

• 出版日期2008-10