Adipogenic Differentiation Is Impaired in Replicative Senescent Human Subcutaneous Adipose-Derived Stromal/Progenitor Cells

作者：Mitterberger Maria C; Lechner Stefan; Mattesich Monika; Zwerschke Werner^*

来源：Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 2014, 69(1): 13-24.

DOI：10.1093/gerona/glt043

• Adipogenesis
• Adipokine
• Adipose-derived stromal/progenitor cells
• Aging
• C/EBP alpha
• Senescence
• Proliferation
• PPAR gamma 2
• p16Ink4A
• p53
• p21Cip1
• Retinoblastoma protein

We demonstrate that adipose-derived stromal/progenitor cells isolated from abdominal subcutaneous fat pads of adult donors successively enter replicative senescence after long-term cultivation. This is characterized by enlarged cell size, flattened morphology, and upregulated senescence-associated beta-galactosidase activity. Moreover, the senescence-associated cyclin-dependent kinase inhibitors p16(Ink4A) and p21(Cip1) were induced correlating with activation of the G1/S cell cycle inhibitor retinoblastoma protein and terminal proliferation arrest. The number of cells in the adipose-derived stromal/progenitor cell population with high adipogenic capacity declined inversely with the increase of senescent cells. Adipogenic hormone cocktail induced expression of the adipogenic key regulators peroxisome proliferator-activated receptor-gamma 2 and CCAAT/enhancer-binding protein alpha was significantly reduced in senescent adipose-derived stromal/progenitor cells. Furthermore, the expression of the adipogenic differentiation genes fatty acid binding protein-4, adiponectin, and leptin and the formation of fat droplets were impaired. We conclude cellular senescence contributes to dysfunctions in adipose-derived stromal/progenitor cell replication, adipogenesis, triglyceride storage, and adipokine secretion.

• 出版日期2014-1