A Fluorescent Tile DNA Diagnocode System for In Situ Rapid and Selective Diagnosis of Cytosolic RNA Cancer Markers

作者：Park Kyung Soo; Shin Seung Won; Jang Min Su; Shin Woojung; Yang Kisuk; Min Junhong; Cho Seung Woo; Oh Byung Keun; Bae Jong Wook; Jung Sunghwan; Choi Jeong Woo^*; Um Soong Ho

来源：Scientific Reports, 2015, 5(1): 18497.

DOI：10.1038/srep18497

Accurate cancer diagnosis often requires extraction and purification of genetic materials from cells, and sophisticated instrumentations that follow. Otherwise in order to directly treat the diagnostic materials to cells, multiple steps to optimize dose concentration and treatment time are necessary due to diversity in cellular behaviors. These processes may offer high precision but hinder fast analysis of cancer, especially in clinical situations that need rapid detection and characterization of cancer. Here we present a novel fluorescent tile DNA nanostructure delivered to cancer cytosol by employing nanoparticle technology. Its structural anisotropicity offers easy manipulation for multifunctionalities, enabling the novel DNA nanostructure to detect intracellular cancer RNA markers with high specificity within 30 minutes post treatment, while the nanoparticle property bypasses the requirement of treatment optimization, effectively reducing the complexity of applying the system for cancer diagnosis. Altogether, the system offers a precise and rapid detection of cancer, suggesting the future use in the clinical fields.

• 出版日期2015-12-18
• 单位Virginia Tech; 美国弗吉尼亚理工大学(Virginia Tech)