A separable surface-enhanced Raman scattering substrate modified with MIL-101 for detection of overlapping and invisible compounds after thin-layer chromatography development

作者：Zhang, Bin bin; Shi, Yi; Chen, Hui; Zhu, Qing xia; Lu, Feng^*; Li, Ying wei

来源：Analytica Chimica Acta, 2018, 997: 35-43.

DOI：10.1016/j.aca.2017.10.006

• Surface-enhanced Raman spectroscopy
• Thin-layer chromatography
• Metal-organic framework-modified gold nanoparticles
• Overlapping and invisible compounds
• Enhanced sensitivity
• Long shelf life

By coupling surface-enhanced Raman spectroscopy (SERS) with thin-layer chromatography (TLC), a powerful method for detecting complex samples was successfully developed. However, in the TLC-SERS method, metal nanoparticles serving as the SERS-active substrate are likely to disturb the detection of target compounds, particularly in overlapping compounds after TLC development. In addition, the SERS detection of compounds that are invisible under both visible light and UV254/365 after TLC development is still a significant challenge. In this study, we demonstrated a facile strategy to fabricate a TLC plate with metal-organic framework-modified gold nanoparticles as a separable SERS substrate, on which all separated components, including overlapping and invisible compounds, could be detected by a point-by-point SERS scan along the developing direction. Rhodamine 6G (R6G) was used as a probe to evaluate the performance of the substrate. The results indicated that the substrate provided good sensitivity and reproducibility, and optimal SERS signals could be collected in 5 s. Furthermore, this new substrate exhibited a long shelf life. Thus, our method has great potential for the sensitive and rapid detection of overlapping and invisible compounds in complex samples after TLC development.

• 出版日期2018-1-2
• 单位中国人民解放军第二军医大学; 上海医药工业研究院; 吉林大学; 华南理工大学; 上海交通大学