Activated H-ras gene mutations in transitional cell carcinoma of urinary bladder in a Kashmiri population

作者：Pandith Arshad Ahmad; Shah Zafar; Rasool Roohi; Dil Afroze; Yousuf Adfar; Parveen Nighat; Wani Saleem; Siddiqi Mushtaq^*

来源：Tumori, 2010, 96(6): 993-998.

• cystectomy
• H-ras
• Kashmir
• PCR-SSCP
• transitional cell carcinoma

Aims and background. The primary aim of the study was to evaluate the incidence of H-ras specific point mutations among a group of Kashmiri patients diagnosed with bladder cancer. We also explored the correlation of clinic-pathological status of the illness with these mutations.
Methods and study design. The DNA samples of both tumor and normal tissue were evaluated for the occurrence of H-ras activating mutations in exon 1 and 2 by PCR-SCCP and DNA sequencing. In addition, blood was also collected from all the cases to rule out any germ-line mutation.
Results. Point mutations of activated H-ras identified in bladder cancer patients were 14.5% (7 of 48), including four transversions (two G -> T and two A -> T) and three transitions (A -> G). Of the mutations, 71.4% were detected in codon 61 and 28.6% in codon 12. The pattern of mutation in the study showed a significant association with smoking in bladder tumors (P < 0.05). No correlation was found between tumor grade and/or stage and the presence of H-ras mutation.
Conclusions. Activation of H-ras by mutation plays a less frequent role than other genetic events in the development of the most transitional cell tumors of the bladder in Kashmiri population. Free full text available at www.tumorionline.it

• 出版日期2010-12
• 单位河北医科大学