科研之友
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摘要
ContextLitsea cubeba (Lour.) Pers. (Lauraceae) has long been used as a folk remedy in Traditional Chinese Medicine (TCM) for the treatment of rheumatic diseases. Previous studies from our laboratory indicated that L. cubeba extract showed anti-arthritic activity in rats.Objective To study L. cubeba chemically and biologically and to find the potential constituents responsible for its anti-arthritic effect.Materials and methods The compounds were isolated from the root of L. cubeba by column chromatography which eluted with PE:EtOAc gradient system, and the structures were elucidated by detailed spectroscopic data analysis; the anti-inflammatory activity of the isolated compounds was evaluated by lipopolysaccharide (LPS)-induced RAW 264.7 cells and the TNF- and NO level were measured by ELISA (commercial kit); The iNOS and COX-2 mRNA expression were measured by RT-PCR and the phosphorylation of IB, IKK, P38 and Akt were determined by western blots.Results A novel 9-fluorenone, 1-ethoxy-3,7-dihydroxy-4,6-dimethoxy-9-fluorenone (1), together with 4 known compounds, namely pinoresinol (2), syringaresinol (3), 9,9-O-di-(E)-feruloyl-meso-5,5-dimethoxysecoisolariciresinol (4) and lyoniresinol (5) were isolated from the root of L. cubeba for the first time. The IC50 for NO inhibition on compounds 1 and 4 were 56.11.2 and 32.8 +/- 2.3M, respectively. The IC50 for TNF- inhibition were 28.2 +/- 0.9 and 15.0 +/- 1.0M, respectively. Both 1 and 4 suppress mRNA expression of iNOS, COX-2 and protein phosphorylation of IB, IKK in LPS-induced RAW 264.7 cells.Discussion and conclusion Compounds 1 and 4 isolated from L. cubeba exhibited potent anti-inflammatory activity through the NF-B signal pathway.
- 出版日期2016-9
- 单位上海中医药大学; 中国人民解放军第二军医大学; 中国人民解放军南京军区福州总医院
