Development and validation of a method based on a QuEChERS procedure and heart-cutting GC-MS for determination of five mycotoxins in cereal products

作者：Cunha Sara C^*; Fernandes Jose O

来源：Journal of Separation Science, 2010, 33(4-5): 600-609.

DOI：10.1002/jssc.200900695

• Deans Switch
• Deoxynivalenol (DON)
• Heart-cutting GC-MS
• QuEChERS
• Trichothecenes
• Zearelenone (ZON)

A new analytical method for the rapid and simultaneous determination of five mycotoxins (zearelenone, deoxynivalenol, Fusarenon X, 15-acetyldeoxynivalenol and nivalenol) in breakfast cereals and flours by heart-cutting GC-MS has been developed and validated. Extraction was performed with MeCN, applying a modified QuEChERS (QUick, Easy, CHeap, Effective, Rugged and Safe) procedure, and the extracts were analyzed after a silylation of the analytes under study. Careful optimization of the parameters of Deans Switch device and GC-MS was achieved in order to attain a fast separation in SIM mode, allowing a total run time of only 8 min. Acceptable recoveries for all mycotoxins at two different spiking levels (20 and 100 mu g/kg) were achieved with good repeatability (from 9 to 21%). LOD ranged from 2 to 15 mu g/kg and LOQ ranged from 5 to 50 mu g/kg. which were lower than the maximum limit legal established by the European Union (EU). The method developed was applied to commercial breakfast cereals and flours; among the mycotoxins studied, deoxynivalenol and zearalenone were the most predominant.

• 出版日期2010-3