科研之友
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摘要
Purpose: This pilot study was aimed to demonstrate the clinical feasibility of using hyperspectral computed tomographic spectroscopy to measure blood oxygen content in human retinal vessels.
Methods: All procedures were performed under a University of Southern California Institutional Review Board-approved protocol and after obtaining informed consent. Fifty-seven subjects with and without diabetic retinopathy were dilated for standard fundus photography. Fundus photographs and retinal vascular oxygen measurements (oximetry) were made using a custom-made hyperspectral computed tomographic imaging spectrometer coupled to a standard fundus camera. Oximetry measurements were made along arteries (A(ox)) and veins (V-ox) within vessel segments that were 1 to 2 disk diameters from the optic disk.
Results: For all control subjects (n = 45), mean A(ox) and V-ox were 93 +/- 7% and 65 +/- 5% (P = 0.001), respectively. For all diabetic subjects (n = 12), mean A(ox) and V-ox were 90 +/- 7% and 68 +/- 5% (P = 0.001), respectively. In subjects with proliferative diabetic retinopathy, A(ox) was significantly lower, and V-ox was significantly higher than other groups (85 +/- 4% and 71 +/- 4%, respectively; P = 0.04, analysis of variance). There was a highly significant difference in the arteriovenous difference between subjects with proliferative diabetic retinopathy and those in the control group (14 vs. 26%, P = 0.003).
Conclusion: Hyperspectral computed tomographic spectroscopy is a clinically feasible method for measurement and analysis of vascular oxygen content in retinal health and disease. This study uses the techniques relevant to oximetry; however, the breadth of spectral data available through this method may be applicable to study other anatomical and functional features of the retina in health and disease.
- 出版日期2014-9
