Activation of Notch-1 in oral epithelial cells by P. gingivalis triggers the expression of the antimicrobial protein PLA(2)-IIA

作者：Al Attar Ahmad; Alimova Yelena; Kirakodu Sreenatha; Kozal Anastasia; Novak Michael John; Stromberg Arnold J; Orraca Luis; Gonzalez Martinez Janis; Martinez Melween; Ebersole Jeffrey L; Gonzalez Octavio A^*

来源：Mucosal Immunology, 2018, 11(4): 1047-1059.

DOI：10.1038/s41385-018-0014-7

P. gingivalis (Pg) is an oral pathogen with the ability to induce oral dysbiosis and periodontal disease. Nevertheless, the mechanisms by which mucosal responses to the oral microbiota in the presence of specific pathogens such as Pg could abrogate the hostmicrobe symbiotic relationship leading to periodontitis remain unclear. Herein, we identified the Notch-1/PLA(2)-IIA axis as a new molecular pathway through which Pg could be specifically modulating oral epithelial antimicrobial and inflammatory responses. Pg activated Notch-1, and inhibition or silencing of Notch-1 completely abrogated Pg-induced PLA(2)-IIA in oral epithelial cells (OECs). Activation of Notch-1 and PLA(2)-IIA production were associated with Pg-produced gingipains. Other oral Gram-positive and Gram-negative species failed to induce similar responses. Pg enhanced OEC antimicrobial activity through PLA(2)-IIA. Increased Notch-1 activation correlated with higher PLA(2)-IIA gingival expression and changes in the abundance of specific oral bacteria phyla during periodontal disease. Oral bacterial species exhibited differential antimicrobial susceptibility to PLA(2)-IIA. These findings support previous evidence suggesting an important role for epithelial Notch-1 activation and PLA(2)-IIA production during health and disease at mucosal surfaces, and provide new mechanistic information concerning the regulation of epithelial antimicrobial and pro-nflammatory responses modulated by oral pathogenic bacteria associated with periodontal disease.

• 出版日期2018-7