Altered Expression of Genes Encoding Cornulin and Repetin in Atopic Dermatitis

作者：Trzeciak Magdalena; Sakowicz Burkiewicz Monika; Wesserling Martyna; Glen Jolanta; Dobaczewska Daria; Bandurski Tomasz; Nowicki Roman; Pawelczyk Tadeusz

来源：International Archives of Allergy and Immunology, 2017, 172(1): 11-19.

DOI：10.1159/000453452

<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; It is assumed that beside alterations in the filaggrin gene (&lt;i&gt;FLG&lt;/i&gt;), disturbances within genes encoding other cornified envelope proteins are also involved in atopic dermatitis (AD). To identify new potential markers of AD, we studied the polymorphisms of genes encoding repetin (&lt;i&gt;RPTN&lt;/i&gt;), cornulin (&lt;i&gt;CRNN&lt;/i&gt;), and their expression in the skin of AD patients. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Polymorphisms in &lt;i&gt;CRNN &lt;/i&gt;(rs941934), &lt;i&gt;RPTN&lt;/i&gt; (rs284544, rs28441202, rs3001978, and rs12117644), and &lt;i&gt;FLG&lt;/i&gt; mutations (R2447X, S3247X) were analyzed by TaqMan genotyping assay and by PCR-RFLP in the blood samples of 159 AD patients and 108 healthy subjects. The expression levels of &lt;i&gt;CRNN&lt;/i&gt; and &lt;i&gt;RPTN&lt;/i&gt; were determined by qRT-PCR in 34 AD skin samples (17 lesional and 17 nonlesional) and in 27 skin biopsies from healthy volunteers. The AD patients were recruited from the clinic of the university hospital between 2012 and 2014. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt;&lt;i&gt;CRNN&lt;/i&gt; rs941934 (A allele) was associated with AD (OR 2.095, &lt;i&gt;p&lt;/i&gt; = 0.008), a severe course of disease (&lt;i&gt;p&lt;/i&gt; = 0.041), elevated IgE levels (&lt;i&gt;p&lt;/i&gt; = 0.047), eosinophilia (&lt;i&gt;p&lt;/i&gt; = 0.018), and concomitant asthma (&lt;i&gt;p&lt;/i&gt; = 0.004). The mRNA level of &lt;i&gt;CRNN&lt;/i&gt; was decreased in the AD skin (&lt;i&gt;p&lt;/i&gt; = 0.041). In the AD patients without &lt;i&gt;FLG&lt;/i&gt; mutations, the CC genotype of &lt;i&gt;RPTN&lt;/i&gt; rs3001978 was associated with AD (OR 0.39, &lt;i&gt;p&lt;/i&gt; = 0.037), early age at onset (&lt;i&gt;p&lt;/i&gt; = 0.033), pruritus (&lt;i&gt;p&lt;/i&gt; = 0.021), severity of AD (&lt;i&gt;p&lt;/i&gt; = 0.045), and concomitant asthma (&lt;i&gt;p&lt;/i&gt; = 0.041). The elevated mRNA levels of &lt;i&gt;RPTN&lt;/i&gt; in lesional (&lt;i&gt;p&lt;/i&gt; &lt; 0.001) and nonlesional (&lt;i&gt;p&lt;/i&gt; = 0.017) AD skin were observed. &lt;b&gt;&lt;i&gt;Conclusions:&lt;/i&gt;&lt;/b&gt; The single-nucleotide polymorphisms of &lt;i&gt;CRNN&lt;/i&gt; (rs941934) and &lt;i&gt;RPTN&lt;/i&gt; (rs3001978, rs28441202) may contribute to AD development, but further studies on a larger group of AD patients are needed to verify this assumption.</jats:p>

• 出版日期2017