Aberrant genes promoter methylation in neural crest-derived tumors

作者：la Torre Annamaria; Muscarella Lucia Anna^*; Parrella Paola; Balsamo Teresa; Bisceglia Michele; Valori Vanna Maria; la Torre Antonella; Barbano Raffaela; Perrella Eleonora; Poeta Maria Luana; Melchionda Gennaro; Merla Giuseppe; Maiello Evaristo; Pellicano Riccardo; Fazio Vito Michele

来源：International Journal of Biological Markers, 2012, 27(4): E389-E394.

DOI：10.5301/JBM.2012.9766

• MCAM
• Methylation
• Neural crest-derived tumors
• RASSF1A

Disturbances in the epigenetic landscape by aberrant methylation of CpG islands can lead to inactivation of cancer-related genes in solid tumors. We analyzed the promoter methylation status of 6 genes previously reported as cancer-specific methylated (MCAM, SSBP2, NISCH, B4GALT1, KIF1A and RASSF1A) in 38 neural crest-derived tumors by quantitative methylation-specific real-time PCR (QMSP). The results demonstrated that the determination of the methylation status of RASSF1A is able to distinguish between normal and tumor samples in cutaneous melanomas, lung carcinoids and small bowel carcinoids. MCAM methylation levels were significantly higher in lung carcinoids tumors (p=0.001), suggesting that this alteration may represent a molecular biomarker in this tumor type.

• 出版日期2012-12