Morphological and Physicochemical Characterization of Agglomerates of Titanium Dioxide Nanoparticles in Cell Culture Media

作者：Freyre Fonseca Veronica; Tellez Medina Dario I; Medina Reyes Estefany I; Cornejo Mazon Maribel; Lopez Villegas Edgar O; Alamilla Beltran Liliana; Ocotlan Flores Jose; Chirino Yolanda I; Gutierrez Lopez Gustavo F^*

来源：Journal of Nanomaterials, 2016, 2016: 5937932.

DOI：10.1155/2016/5937932

Titanium dioxide nanoparticles (TiO2 NP) are possible carcinogenic materials (2B-IARC) and their toxicity depends on shape, size, and electrical charge of primary NP and on the system formed by NP media. The aim of this work was to characterize agglomerates of three TiO2 NP by evaluating their morphometry, stability, and zeta potential (zeta) in liquid media and their changes with time. Sizes of agglomerates by dynamic light scattering (DLS) resulted to be 10-50 times larger than those obtained by digital image analysis (DIA) given the charged zone around particles. Fractal dimension (FD) was highest for agglomerates of spheres and belts in F12K, and in E171 in FBS media. E171 and belts increased FD with time. At time zero, using water as dispersant FD was larger for agglomerates of spheres than for of E171. Belts suspended in water had the smallest values of circularity (Ci) which was approximately unchanged with time. All dispersions had zeta values around -30mV at physiological pH (7.4) and dispersions of NP in water and FBS showed maximum stability (Turbiscan Lab analysis). Results help in understanding the complex NP geometry-size-stability relationships when performing in vivo and in vitro environmental-toxicity works and help in supporting decisions on the usage of TiO2 NP.

• 出版日期2016