Phage as versatile nanoink for printing 3-D cell-laden scaffolds

作者：Lee Doe Young; Lee Hyeongjin; Kim YongBok; Yoo So Young; Chung Woo Jae^*; Kim GeunHyung

来源：Acta Biomaterialia, 2016, 29: 112-124.

DOI：10.1016/j.actbio.2015.10.004

• Phages
• Bioprinting
• Bioinks
• 3-D scaffolds
• Tissue engineering
• Genetic engineering

Bioprinting is an emerging technology for producing tissue-mimetic 3-D structures using cell-containing hydrogels (bioink). Various synthetic and natural hydrogels with key characteristics, including biocompatibility, biodegradability, printability and crosslinkability, have been employed as ink materials in bioprinting. Choosing the right cell-containing "bioink" material is the most essential step for fabricating 3-D constructs with a controlled mechanical and biochemical microenvironment that can lead to successful tissue regeneration and repair. Here, we demonstrate that the genetically engineered M13 phage holds great potential for use as a versatile nanoink for printing 3-D cell-laden matrices. In particular, M13 phages displaying integrin-binding (GRGDS) and calcium-binding (DDYD) domains on their surface were blended with alginate to successfully form Ca2+-crosslinked hydrogels. Furthermore, 3-D cell-laden scaffolds with high cell viability were generated after optimizing the printing process. The MC3T3-E1 cells within these scaffolds showed enhanced proliferation and differentiation rates that increased proportionally with the concentration of phages in the 3-D matrices compared with the rates of cells in pure alginate scaffolds.
Statement of significance
Bioprinting is an emerging technology for producing tissue-mimetic 3-D structures using cell-containing hydrogels called bioink. Choosing the right bioink is essential for fabricating 3-D structures with controlled mechanical and biochemical properties which lead to successful tissue regeneration. Therefore, there is a growing demand for a new bioink material that can be designed from molecular level. Here, we demonstrate that genetically engineered M13 phage holds great potential for use as versatile bioink. The phage-based bioink benefits from its replicability, self-assenibling property, and tunable molecular design and enables bioprinted scaffolds to exhibit improved cell viability, proliferation and differentiation. This study opens the door for the development of genetically tunable nanofibrous bioink materials which closely mimic natural structural proteins in the extracellular matrix.

• 出版日期2016-1-1