Voltage-gated calcium channels in the human adrenal and primary aldosteronism

作者：Felizola Saulo J A; Maekawa Takashi; Nakamura Yasuhiro^*; Satoh Fumitoshi; Oho Yoshikiyo; Kikuchi Kumi; Aritomi Shizuka; Ikeda Keiichi; Yoshimura Michihiro; Tojo Katsuyoshi; Sasano Hironobu

来源：Journal of Steroid Biochemistry and Molecular Biology, 2014, 144: 410-416.

DOI：10.1016/j.jsbmb.2014.08.012

• Calcium channel
• Aldosterone
• Adrenal cortex
• Aldosterone producing adenoma
• Immunohistochemistry

Calcium channel blockers can efficiently be used in the treatment of primary aldosteronism (PA) related hypertension, but details on the localization of calcium channel (CC) in the human adrenal and its disorders, including PA, have remained unclear. Therefore, in this study we analyzed the known alpha subunits of L-, N- and T-type CCs in 74 adrenocortical aldosterone-producing adenomas (APA) and 16 cortisol-producing adenomas (CPA) using quantitative RT-PCR (qPCR). We also examined the status of L-(CaV1.2, CaV1.3), N-(CaV2.2) and T-(CaV3.2) CC subunits in five non-pathological adrenals (NA), five idiopathic hyperaldosteronism (IHA) cases, and 50 APA using immunohistochemistry. After qPCR evaluation, only CaV1.2, CaV1.3, CaV2.2, and CaV3.2 mRNA levels could be detected in APA and CPA. Among those, only CaV3.2 mRNA levels were significantly correlated with plasma aldosterone levels (P = 0.0031), CYP11B2 expression levels (P %26lt; 0.0001) and the presence of KCNJ5 mutations (P = 0.0019) in APA. The immunolocalization of CCs in NA and IHA was detected in the zona glomerulosa (ZG), with a predominance of CaV3.2 in APA. These findings suggest that different types of CC can be involved in calcium-related aldosterone biosynthesis.

• 出版日期2014-10