Association of novel polymorphisms in the bovine myocyte enhancer factor 2D (MEF2D) gene with carcass traits of Polish Holstein-Friesian cattle

作者：Juszczuk Kubiak E^*; Wicinska K; Oprzadek J

来源：Czech Journal of Animal Science, 2013, 58(6): 262-269.

• MEF2D gene
• gene polymorphism
• combined genotype
• meat contents
• bulls

In the present study the polymorphism of the myocyte enhancer factor 2D (MEF2D) gene was analyzed as a genetic marker candidate for carcass traits in Polish Holstein-Friesian cattle. Four novel single nucleotide polymorphisms (SNPs): HQ692911: g. 93C>T in exon 8, JX088659: g. 69C>T in intron 8, JX0692914: g. 47C>T in intron 9 as well as JX088658: g. 100G>A in the 3'flanking region were identified by DNA sequencing. By applying the PCR-RFLP method, the SNPs (g. 93C>T/HaeIII and g. 47C>T/Tsp45I) were genotyped in 401 Polish Holstein-Friesian bulls and their association with carcass traits was analyzed. Statistical analysis showed that the g. 93C>T SNP was significantly associated with weight of lean (WLVC) and fat in valuable cuts (WFVC). Animals with the TT genotype had significantly higher WLVC (P < 0.05) and lower WFVC (P < 0.05) than those with the CC and CT genotypes. No significant relationship with carcass traits was found for the g. 47C>T SNP (P > 0.05) in intron 9. Moreover, we found a significant effect of the combined genotypes on WFVC (P < 0.01), percent of lean (PLVC) (P < 0.05), and fat (PFVC) in valuable cuts (P < 0.01). This suggested that MEF2D is a strong candidate gene that affects carcass traits in cattle.

• 出版日期2013