Multiple target loci assembly sequencing (mTAS)

作者：Han Hyojun; Yoon Jung ki; Cho Byoung Chul; Kim Hwangbeom; Bang Duhee^*

来源：Analytical Biochemistry, 2011, 415(2): 218-220.

DOI：10.1016/j.ab.2011.04.012

Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis.

• 出版日期2011-8-15