Accumulation of Cadmium by Transgenic Tobacco Plants (Nicotiana tabacum L.) Carrying Yeast Metallothionein Gene Revealed by Electrochemistry

作者：Krystofova Olga^*; Zitka Ondrej; Krizkova Sona; Hynek David; Shestivska Violetta; Adam Vojtech; Hubalek Jaromir; Mackova Martina; Macek Tomas; Zehnalek Josef; Babula Petr; Havel Ladislav; Kizek Rene

来源：International Journal of Electrochemical Science, 2012, 7(2): 886-907.

• Metallothionein
• Tobacco
• Nicotiana tabacum
• Thiols
• Phytoremediation
• Electrochemical detection
• Brdicka reaction
• Voltametry
• Liquid chromatography
• Heavy metal

Phytoremediation of heavy metals includes phytoextraction and phytoaccumulation, i.e. accumulation of pollution from soils or water into the plants. Inorganic pollutants like heavy metals cannot be chemically degraded and thus it has to be accumulated in roots and leaves of the plants in non-toxic forms. In this study, the influence of cadmium on transgenic Nicotiana tabacum plants carrying yeast metallothionein was investigated. Tobacco plants were exposed to 0, 50, 100 and 250 mu M CdCl2 for 96 hours. Growth parameters as well as changes in content of cysteine, oxidized/reduced glutathione, phytochelatins (PC2, PC3 and PC4) were monitored using high performance liquid chromatography with electrochemical detection. Moreover, we exploited differential pulse voltammetry Brdicka reaction for determination of metallothionein and verified the results obtained by dot blot assay. In addition, all results were compared with results obtained using non-transgenic tobacco plants. Our results indicate protective role of metallothionein as well as its role in cadmium accumulation in roots of transgenic plants.

• 出版日期2012-2