Genetic diversity and phylogenetic relationship of Tadehagi in southwest China evaluated by inter-simple sequence repeat (ISSR)

作者：Liu, Diqiu; He, Xin; Liu, Guodao; Huang, Bizhi^*

来源：Genetic Resources and Crop Evolution, 2011, 58(5): 679-688.

DOI：10.1007/s10722-010-9611-3

• Genetic diversity
• Inter-simple sequence repeat
• ISSR
• Phylogenetic relationship
• Tadehagi

There are many valuable Tadehagi accessions in southwest China, but it is unknown that the genetic diversity and phylogenetic relationship of these Tadehagi resources. This report is the first study in which 41 primers of inter-simple sequence repeat (ISSR) were used to assess the genetic diversity of 36 Tadehagi accessions from 3 provinces in the southwest of China. Totally, 30 usable ISSR primers detected 163 polymorphic bands among the 36 accessions, which suggested high utility of ISSR primers in the genetic analysis of Tadehagi accessions. Genetic similarity coefficients among all of the accessions ranged from 0.54 to 0.92 with the average of 0.79 based on the ISSR data, indicating high level of genetic variation in Tadehagi resources from the southwest of China. As for the 3 population, Hainan population had the maximum average genetic similarity coefficients of 0.81, while similarity coefficient of Guangxi and Yunnan population was 0.75 and 0.74, respectively. All the 36 Tadehagi accessions were divided into 4 groups in the UPGMA dendrogram constructed from genetic similarity coefficients. The Tadehagi accessions from Yunnan and Guangxi provinces showed more genetic variation and occupied the bottom of the dendrogram. On the contrary, those from Hainan Province had less genetic variation and clustered in the middle and top of the dendrogram. The information on the genetic diversity and phylogenetic relationship from this study is propitious to construct a core germplasm collection and develop novel Tadehagi cultivars with desired economic traits.

• 出版日期2011-6
• 单位云南省草地动物科学研究院; 昆明理工大学; 中国热带农业科学院