A Study of Immunochromatographic Fecal Occult Blood Test Methods

作者：Wang, Sen; Wang, Xian; Tang, Shengnan; Chen, Yuxin; Jia, Jia; Chen, Junhao; Shen, Han; Bai, Bing^*

来源：Laboratory Medicine, 2019, 50(2): 130-137.

DOI：10.1093/labmed/lmy057

• FOB
• Triton X-100
• immunochromatography
• hemoglobin
• digestive tract cancer
• detergent

Background The sensitivity of the commonly used immunochromatographic fecal occult blood test (iFOBT) is critical in the routine screening of digestive tract cancers. @@@ Objective To determine whether the performance of the iFOBT might be improved by optimizing detergents and buffers in stool solution. @@@ Methods We tested different buffers and detergents in stool solution. The specificity of the new solution condition was confirmed by dot immunoblotting. A total of 1122 clinical stool specimens were collected to evaluate the clinical application of this refined stool solution. @@@ Results The different salts exerted minor effects; also, sodium dodecyl sulfate (SDS) prevented the test from working as designed. However, 1% Triton X-100 improved the sensitivity significantly, as confirmed by dot immunoblot testing. With 1% Triton X-100 in the stool solution, the rate of positive iFOBT results in patients with digestive tract cancers increased, from 40.4% to 49.4%. @@@ Conclusion Use of 1% Triton X-100 improves the sensitivity of the iFOBT and thus, this substance should be routinely included in iFOBT for screening of digestive tract cancers.

• 出版日期2019-4
• 单位南京大学