A DICTYOSTELIUM-DISCOIDEUM GENE, WHICH IS HIGHLY RELATED TO MO15 FROM XENOPUS, IS EXPRESSED DURING GROWTH BUT NOT DURING DEVELOPMENT

作者：MICHAELIS C^*; LUO QA; WEEKS G

来源：Biochemistry and Cell Biology, 1995, 73(1-2): 51-58.

• CELL CYCLE
• DIFFERENTIATION
• CELL TYPE DETERMINATION
• CDC2 KINASE
• DICTYOSTELIUM
• MO15 PROTEIN

We have isolated a cDNA from the cellular slime mold Dictyostelium discoideum encoding a protein that is 52% identical to the Xenopus Mo15 kinase and highly related to the equivalent proteins from human (52% identity), rice (52.7% identity), and yeast (47.6% identity). Mo15 is responsible for the activation of Cdc2 kinase and is itself a member of the large Cdc2-related family of protein kinases. The Dictyostelium protein is more related to the Xenopns Mo15 protein than it is to either the Dictyostelium Cdc2 or Crp proteins. Southern blot analysis of genomic V12-M2 DNA indicated that mo15 is present as a single copy gene that cross hybridizes with cdc2 at low stringency. Northern blot analysis of RNA from different stages of Dictyostelium development showed that mo15 is only expressed during vegetative cell growth.

• 出版日期1995-2