Antioxidant activity and Inhibitory potential of few Indian medicinal plants against key hyperglycemic enzymes

作者：Mala Majumdar^*

来源：Research Journal of Biotechnology, 2012, 7(4): 186-191.

• In vitro study
• Antioxidant activity
• DPPH and Iron chelation
• Hypoglycemic potential

This study evaluated the in vitro antioxidant potential and inhibition of alpha-amylase and alpha-glucosidase activities of methanolic extracts of leaves of few Indian medicinal plants. The antioxidant activities of the samples were measured by two different methods while the Folin-Ciocalteu reagent assay was used to estimate the phenolic contents of extracts. Methanolic extracts from dried leaves of P. emblica, C. cajan, C. indica and F. pratensis were prepared. Antioxidant activities were estimated by DPPH radical scavenging and iron chelating methods. The inhibition of carbohydrate-hydrolysing key enzymes e. g. alpha-amylase and alpha-glucosidase activities were assayed. Acarbose was used as reference compound in both the assays.
Experimental results are presented as the mean+/-(SEM) of triplicates. Following order C. indica >= C. cajan > F. pratensis > P. emblica was observed with both DPPH and iron chelating methods. Observed a amylase inhibitory effect was F. pratensis (74.9%), C. indica(59.52%), P. emblica (55.33%) and C. cajan (48.74%) at a concentration of 200 mu g/ml. C. indica (86.9%) exhibited highest alpha-glucosidase inhibition followed by C. cajan(70.23%), F. pratensis(26.19%) and P. emblica(5.95%). The inhibition of these enzymes can lead to lower postprandial blood glucose. Based on the results, C. indica exhibiting high antioxidant and enzyme inhibition should be further investigated to identify bioactive compounds.

• 出版日期2012-11