Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae

作者：Suzuki Toshihiro; Sakamoto Takatoshi; Sugiyama Minetaka; Ishida Nobuhiro; Kambe Hiromi; Obata Shusei; Kaneko Yoshinobu; Takahashi Haruo; Harashima Satoshi^*

来源：Journal of Bioscience and Bioengineering, 2013, 115(5): 467-474.

DOI：10.1016/j.jbiosc.2012.11.014

• Saccharomyces cerevisiae
• Lactic-acid resistance
• L-Lactic-acid production
• Multiple disruption
• Genome-wide screening

To create strains that have high productivity of lactic acid without neutralization, a genome-wide screening for strains showing hyper-resistance to 6% L-lactic acid (pH 2.6) was performed using the gene deletion collection of Saccharomyces cerevisiae. We identified 94 genes whose disruption led to resistance to 6% lactic acid in rich medium. We also found that multiple combinations of Delta dse2, Delta scw11, Delta eaf3, and/or Delta sed1 disruption led to enhanced resistance to lactic acid depending upon their combinations. In particular, the quadruple disruptant Delta dse2 Delta scw11 Delta eaf3 Delta sed1 grew well in 6% lactic acid with the shortest lag phase. We then introduced an exogenous lactate dehydrogenase gene (LDH) into those single and multiple disruptants to evaluate their productivity of lactic acid. It was found that the quadruple disruptant displaying highest lactic-acid resistance showed a 27% increase of lactic-acid productivity as compared with the LDH-harboring wild-type strain. These observations suggest that disruption of multiple genes whose deletion leads to lactic-acid resistance is an effective way to enhance resistance to lactic acid, leading to high lactic-acid productivity without neutralization.

• 出版日期2013-5