A novel Alcohol Biosensor Based on Alcohol Dehydrogenase and Modified Electrode with ZrO2 Nanoparticles

作者：Salimi Farough^*; Negahdary Masoud; Mazaheri Gholamreza; Akbari dastjerdi Hajar; Ghanbari kakavandi Yousoof; Javadi Sholeh; Inanloo Seyed Hosein; Mirhashemi route Maryam; Shokoohnia Mohammad Hani; Sayad Aida

来源：International Journal of Electrochemical Science, 2012, 7(8): 7225-7234.

• alcohol biosensor
• alcohol dehydrogenase
• Zirconia nanoparticles
• carbon paste electrode

In this study, we developed an electrochemical ADH-based biosensor as a simple and convenient tool for the detection of ethanol. The carbon paste electrode was modified with Zro(2) nanoparticles. Direct electrochemistry of ADH in this paste electrode was easily achieved, and a pair of well-defined quasireversible redox peaks appeared with a formal potential (E-0) of - (450 +/- 1) mV (versus SCE) in pH 7.0 phosphate buffer solution (PBS). The linear range of this biosensor for ethanol determination was from 1 to 26 mM. This novel biosensor showed high sensitivity, a wide linear range, low detection limit and good stability for electrochemical detection of ethanol. This work may represent a facile, cheap and promising approach for the fabrication of various electrochemical biosensors.

• 出版日期2012-8