An Investigation on Heavy Metals Bio-Sorption by Azolla filiculoides in the International Anzali Wetland

作者：Sereshti H^*; Bakhtiari S; Toghyani R; Samadi S; Besharati Seidani T; Mahmoodi N O

来源：International Journal of Environmental Research, 2014, 8(2): 377-386.

• Heavy metals
• Anzali wetland
• Azolla filiculoides
• Experimental design
• ICP-AES

The ultrasound assisted emulsification microextraction (USAEME) method followed by inductively coupled plasma-atomic emission spectrometry (ICP-AES) was used for preconcentration and determination of bismuth (Bi), indium (In) and lead (Pb) in the plant Azolla filiculoides Lam. Calix [4] pyrrole was used as a chelating agent and tetrachloroethylene was selected as extraction solvent. The main parameters of the USAEME method were optimized by using response surface methodology (RSM). The optimal conditions were as follows: 130 mu L for volume of extraction solvent, 4 min for sonication time, 6.75 (w/v %) for salt concentration, 890 mg/L for concentration of chelating agent and 7.5 for pH. The calibration graphs were linear in the range of 1-1500 mu g/L for Bi and In, and 1-1000 mu g/L for Pb with the determination coefficients (R-2) of 0.9983, 0.9974 and 0.9997, respectively. The limits of detection (LODs) for Bi, In and Pb were 0.57, 0.76 and 0.84 mu g/L, respectively. Under the optimal conditions, the method was successfully applied to the determination of Bi, In and Pb in Azolla filiculoides Lam, tissues in Anzali wetland and satisfactory relative recoveries (94-97%) were achieved.

• 出版日期2014