Development of new methods for the quantitative detection and typing of Lactobacillus parabuchneri in dairy products

作者：Berthoud Helene^*; Wuthrich Daniel; Bruggmann Remy; Wechsler Daniel; Frohlich Wyder Marie Therese; Irmler Stefan

来源：International Dairy Journal, 2017, 70: 65-71.

DOI：10.1016/j.idairyj.2016.10.005

Thirty-one isolates of Lactobacillus parabuchneri were obtained from cheese containing histamine; of these, 26 were found to possess the hdcA gene encoding histidine decarboxylase. By analysing the genome data of 13 isolates, specific targets for the development of PCR-based detection and typing systems for L parabuchneri were identified. The real-time PCR for detection showed a linear quantification over a range of 7 logs and a detection limit of 10 gene equivalents per reaction. The strain typing method utilised the amplification of repeat sequences and showed discrimination comparable with a phylogenetic tree, based on genome comparisons. The method was suitable for detecting and monitoring the development of L. parabuchneri in raw milk and cheese.

• 出版日期2017-7