Morphological and allozyme variation in a collection of Cucumeropsis mannii Naudin (Cucurbitaceae) from Cote d'Ivoire

作者：Koffi Kouame K; Gbotto Ahou A; Malice Marie; Dje Yao; Bertin Pierre; Baudoin Jean Pierre; Bi Irie A Zoro^*

来源：Biochemical Systematics and Ecology, 2008, 36(10): 777-789.

DOI：10.1016/j.bse.2008.07.008

• Isozyme variation
• Cucumeropsis mannii
• Cucurbit
• Morphological variability
• Cultivar identification

To set up a rational collecting strategy for germplasm of the edible-seeded cucurbit Cucumeropsis mannii, a study was conducted using 24 morphological and seven putative enzyme markers to determine the intra-specific variability from 16 and 22 accessions (representing three cultivars), respectively. The analysis of variance, showed a significant difference between the three cultivars. Principal component analysis pointed out a variation among individuals, mainly on the basis of flower, fruit, and seed size. Dendrogram with UPGMA method allowed clustering of the cultivars. Genetic diversity indices estimated equalled: 9.96% for the proportion of polymorphic loci (P), 1.10 for the number of alleles (A) and 0.023 for observed heterozygosity (H(o)). The level of the within accessions genetic diversity (H(S)=0.078) was higher than among accessions (D(ST)=0.042). Nei's genetic distances between the three cultivars were also low (0.079-0.147), indicating a high degree of similarity of the analysed cultivars.

• 出版日期2008-10