Detection of infectious dengue virus by selective real-time quantitative polymerase chain reaction

作者：Huang, Xin; Zhou, Xuan; He, Xiaoyan; Wang, Pei; Yue, Shuai; Wu, Lixin; Zhang, Yu; Xie, Qian; Zhang, Bao^*; Zhao, Wei^*

来源：VIROLOGICA SINICA, 2016, 31(4): 342-345.

DOI：10.1007/s12250-016-3757-x

• PROPIDIUM MONOAZIDE
• PCR
• DISTINGUISH
• BACTERIA

<正>Dear Editor,The dengue virus(DENV)is a single-stranded positivesense RNA virus that belongs to the family Flaviviridae(Gubler,2002),and has four serotypes,DENV1–DENV4,which are transmitted via Aedes aegypti and Aedes albopictus(Rodriguez-Roche and Gould,2013).It has been reported that more than 50 million dengue infections occur each year(Guzman et al.,2010),and a serious outbreak occurred in the Southern Provinces of

• 出版日期2016-8
• 单位南方医科大学