Depth-resolved velocimetry of Hagen-Poiseuille and electro-osmotic flow using dynamic phase-contrast microscopy

作者：Esseling Michael^*; Holtmann Frank; Woerdemann Mike; Denz Cornelia

来源：Applied Optics, 2010, 49(31): 6030-6038.

DOI：10.1364/AO.49.006030

We quantitatively investigate the axial imaging properties of dynamic phase-contrast microscopy, with a special focus on typical combinations of tracer particles and magnifications that are used for velocimetry analysis. We show, for the first time, that a dynamic phase-contrast microscope, which is the integration of an all-optical novelty filter in a commercially available inverted microscope, can visualize three-dimensional velocity fields with a significantly reduced optical sectioning depth. The depth of field for dynamic phase-contrast microscopy is extracted from the three-dimensional response function and compared with the respective values for incoherent bright-field illumination. These results are then used to perform a depth-resolved particle image velocimetry analysis of Hagen-Poiseuille as well as electro-osmotically actuated flows in a microchannel.

• 出版日期2010-11-1