Nanodesign of new self-assembling core-shell gellan-transfersomes loading baicalin and in vivo evaluation of repair response in skin

作者：Manconi Maria; Manca Maria Letizia^*; Caddeo Carla; Valenti Donatella; Cencetti Claudia; Diez Sales Octavio; Nacher Amparo; Mir Palomo Silvia; Carmen Terencio Maria; Demurtas Davide; Carmelo Gomez Fernandez Juan; Jose Aranda Francisco; Fadda Anna Maria; Matricardi Pietro

来源：Nanomedicine: Nanotechnology, Biology and Medicine , 2018, 14(2): 569-579.

DOI：10.1016/j.nano.2017.12.001

• Transfersomes
• Gellan
• SAXS analysis
• Skin delivery
• Rheological studies
• In vivo studies

Gellan nanohydrogel and phospholipid vesicles were combined to incorporate baicalin in new self-assembling core-shell gellantransfersomes obtained by an easy, scalable method. The vesicles were small in size (similar to 107 nm) and monodispersed (P.I. <= 0.24), forming a viscous system (similar to 24 mPa/s) as compared to transfersomes (similar to 1.6 mPa/s), as confirmed by rheological studies. Gellan was anchored to the bilayer domains through cholesterol, and the polymer chains were distributed onto the outer surface of the bilayer, thus forming a core-shell structure, as suggested by SAXS analyses. The optimal carrier ability of core-shell gellan-transfersomes was established by the high deposition of baicalin in the skin (similar to 11% in the whole skin), especially in the deeper tissue (similar to 8% in the dermis). Moreover, their ability to improve baicalin efficacy in anti-inflammatory and skin repair tests was confirmed in vivo in mice, providing the complete skin restoration and inhibiting all the studied inflammatory markers.

• 出版日期2018-2