Population survey of spiders using mt-DNA (COI) sequences in Japanese peach orchards

作者：Sonoda Shoji^*; Yamashita Jun; Kohara Yoko; Izumi Yohei; Yoshida Hideya; Enomoto Takashi

来源：Applied Entomology and Zoology, 2011, 46(1): 81-86.

DOI：10.1007/s13355-010-0011-0

• Cytochrome oxidase subunit I
• Mitochondrial DNA
• Molecular phylogeny
• Population surveys
• Spiders

In this study, we attempted a population survey of spiders in peach orchards with and without pesticide application; however, we had difficulty discriminating them. To identify spiders, we determined the nucleotide sequences of a ca. 640-bp region in the mitochondrial cytochrome oxidase subunit I (COI) gene from 17 species belonging to eight families. To identify five wolf spiders (Araneae: Lycosidae), which are the most abundant spiders in peach orchards in Okayama Prefecture, PCR and restriction fragment length polymorphism were developed on the basis of the COI gene sequences. Population survey results suggest that pesticide applications adversely affect spider populations in peach orchards.

• 出版日期2011-2