Two-Site Antibody Immunoanalytical Detection of Food Allergens by Surface Plasmon Resonance

作者：Wu, Xuli^*; Li, Yao; Liu, Bo; Feng, Yue; He, Weiyi; Liu, Zhigang; Liu, Lizhong; Wang, Zimei; Huang, Haizhen^*

来源：Food Analytical Methods, 2016, 9(3): 582-588.

DOI：10.1007/s12161-015-0232-5

• beta-Lactoglobulin
• Ara h1
• SPR
• Food allergen detection

Bovine protein beta-lactoglobulin (beta LG) and peanut protein Ara h1 are considered good targets for detecting milk and peanut allergen, respectively. We used surface plasmon resonance (SPR) to detect beta LG and Ara h1 by immobilizing the affinity-purified monoclonal antibodies on the biosensor chip. Proteins that bound to the antibody surface were detected by a shift in resonance angle. Adding polyclonal antibodies in the sandwich assay enhanced the sensitivity. beta LG and Ara h1 were detected at 5.54 and 0.77 ng/mL, respectively, by SPR, and the results demonstrated good linear relation with relatively low concentrations of protein. The limit of detection with SPR was comparable to that with sandwich enzyme-linked immunosorbent assay (S-ELISA) with the same polyclonal and monoclonal antibodies. Use of the SPR biosensor is a simple, fast, and reliable way to detect beta LG and Ara h1.

• 出版日期2016-3
• 单位深圳大学