A Human Cell Line Model for Interferon-alpha Driven Dendritic Cell Differentiation

作者：Ruben Jurjen M; Visser Lindy L; Heinhuis Kimberley M; O'Toole Tom; Bontkes Hetty J; Westers Theresia M; Ossenkoppele Gert J; de Gruijl Tanja D; van de Loosdrecht Arjan A^*

来源：PLos One, 2015, 10(8): e0135219.

DOI：10.1371/journal.pone.0135219

The CD34(+) MUTZ-3 acute myeloid leukemia cell line has been used as a dendritic cell (DC) differentiation model. This cell line can be cultured into Langerhans cell (LC) or interstitial DC-like cells using the same cytokine cocktails used for the differentiation of their primary counterparts. Currently, there is an increasing interest in the study and clinical application of DC generated in the presence of IFN alpha, as these IFN alpha-DC produce high levels of inflammatory cytokines and have been suggested to be more potent in their ability to cross-present protein antigens, as compared to the more commonly used IL-4-DC. Here, we report on the generation of IFN alpha-induced MUTZ-DC. We show that IFN alpha MUTZ-DC morphologically and phenotypically display characteristic DC features and are functionally equivalent to "classic" IL-4 MUTZ-DC. IFN alpha MUTZ-DC ingest exogenous antigens and can subsequently cross-present HLA class-I restricted epitopes to specific CD8(+) T cells. Importantly, mature IFN alpha MUTZ-DC express CCR7, migrate in response to CCL21, and are capable of priming naive antigen-specific CD8(+) T cells. In conclusion, we show that the MUTZ-3 cell line offers a viable and sustainable model system to study IFN alpha driven DC development and functionality.

• 出版日期2015-8-7