Development of Optical Sugar Sensors as Implantable Devices for Interstitial Glucose Monitoring

作者：Tohda Koji^*

来源：Bunseki Kagaku, 2013, 62(10): 903-914.

DOI：10.2116/bunsekikagaku.62.903

• blood glucose level
• implantable optical glucose monitor
• enzyme biosensor
• synthetic glucose receptor
• competitive complexation

In order to develop implantable devices for the continuous monitoring of interstitial glucose, two kinds of sensors, namely, enzymatic and synthetic receptor-based optical sugar sensors have been fabricated and their response properties evaluated. Enzymatic sensors are based on microscopic pH-sensitive optode beads and glucose oxidase (GOX)-immobilized beads inside hydrophilic membrane capsules with ca. 12 mu m thickness. A theoretical model describing the sensor glucose responses was proposed. In this model, the glucose influx and the gluconic acid efflux across the capsule membrane were combined with the enzymatic kinetics inside the capsule. Excellent agreements of the predicted sensor responses with experimentally obtained ones allowed us to confirm that enhanced sensor responses for glucose could be achieved by reducing the gluconic acid efflux across the capsule membrane. The synthetic receptor-based sensors were fabricated by covalently immobilizing phenylboronic acid receptors and boronate specific alizarin dyes on a hydrogel film. Rapid and reversible absorbance responses for fructose were induced by dissociation of the receptor/dye complexes on the film due to competitive complexation between the receptor and the analyte sugar. In addition, to obtain receptors with higher glucose affinity, a series of his (phenyl boronic acid)-type receptors were designed and synthesized. Among them, it was found that a bis(benzoboroxole)-type receptor possessing a m-xylenediamine linker showed the largest binding constant for glucose.

• 出版日期2013-10