An electrochemical quartz crystal impedance study on anti-human immunoglobulin G immobilization in the polymer grown during dopamine oxidation at an Au electrode

作者：He, H; Xie, QJ^*; Yao, SZ

来源：Journal of Colloid and Interface Science, 2005, 289(2): 446-454.

DOI：10.1016/j.jcis.2005.03.085

• electrochemical quartz crystal impedance analysis
• polymerization during dopamine oxidation
• immobilization of anti-human IgG
• human IgG
• Au electrode

The polymeric film grown during dopamine oxidation at an An electrode was studied as a novel matrix for immobilizing anti-human immunoglobulin G (IgG) via the electrochemical quartz crystal impedance analysis (EQCIA) method. The growth of the polymeric films at Au electrodes during dopamine oxidation in neutral phosphate buffer (pH 7.4) and the immobilization of anti-human I.-G into the polymeric films during their growth have been traced at real time. Lysozyme control experiments suggested that anti-human IgG was electrostatically incorporated into the polymeric film. Also, the porosity of the polymeric films has been discussed by measuring the "wet" and "dry" frequency shifts. Compared with a polypyrrole film immobilized with anti-human IgG, the proposed matrix possessed a larger amount of specific binding sites for human IgG by subsequent immunoreaction tests. The association constant of the anti-human IgG immunoreaction was obtained with satisfactory results.

• 出版日期2005-9-15
• 单位湖南师范大学