A thiazolo [4,5-b]pyridine-based fluorescent probe for detection of zinc ions and application for in vitro and in vivo bioimaging

作者：Gong, Jiao; Li, Yuan-heng; Zhang, Chan-juan; Huang, Jian^*; Sun, Qi^*

来源：Talanta, 2018, 185: 396-404.

DOI：10.1016/j.talanta.2018.03.061

• Thiazolo[4,5-b]pyridine
• Zinc ions
• Fluorescent probe
• Membrane permeable
• Lysosomal selectivity

2-HPTP, a novel thiazolo [4, 5-b] pyridine-based Zn2+ selective fluorescent probe has been synthesized and investigated. This probe exhibited a high selectivity towards Zn2+ over other biologically essential cations such as Na+, K+, Ca2+, or Mg2+. 2-HPTP formed a 1:1 complex with Zn2+ and showed a fluorescent enhancement with a long emission wavelength red-shift (85 nm) upon complex formation with Zn2+. The detection limit and association constant were calculated as 3.48 x 10(-7) M and 2.40 x 10(6) M-1 by a fluorescence titration experiment. Furthermore, the live cell imaging experiment showed that 2-HPTP was membrane permeable and photostable, and hence, could be used to monitor the concentration changes of intracellular Zn2+. The co-staining experiments in the cells demonstrated that 2-HPTP possessed high lysosomal selectivity in living cells. Finally, using the nematode C. elegans as an experimental model, we established that 2-HPTP could be successful in imaging Zn2+ concentration changes in living tissues. Therefore, this molecule should be useful for studies on the biological functions of Zn2+.

• 出版日期2018-8-1
• 单位北京大学